recombinant protein galectin 8 (Sino Biological)

Sino Biological recombinant protein galectin 8
$(A) Data analysis with TIDE shows that LGALS8 plays an important role in T cell dysfunction in tumor microenvironment. (B) Spearman correlations between <t>Galectin-8</t> expression and TILs. (C) TIMER was used to calculated MDSC fraction and correlation with LGALS8expression in the indicated types of tumors from the TCGA dataset. (D) Schematic procedure of phagocytosis assay. (E) Phagocytosis assay (FC) shows that Galectin-8 inhibits the ability of phagocytosis. F. Transcriptome analysis of CD14+ monocytes exposed with Galectin-8. (F) Heatmap of transcriptome sequencing data. Samples of 2 groups including control and gal-8 were analyzed. (G) Volcano plot of transcriptome sequencing data. Significantly regulated genes were labeled.$
Recombinant Protein Galectin 8, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+lgals8/Human+Galectin-8+%2F+LGALS8+Protein/bio_rxiv__2022__07__27__501694-179-2-6
Average 92 stars, based on 1 article reviews

recombinant protein galectin 8 - by Bioz Stars, 2026-09

92/100 stars

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antibody lgals8 mammalian hek293 aaf19370 1 (Sino Biological)

Sino Biological antibody lgals8 mammalian hek293 aaf19370 1
$(A) Data analysis with TIDE shows that LGALS8 plays an important role in T cell dysfunction in tumor microenvironment. (B) Spearman correlations between <t>Galectin-8</t> expression and TILs. (C) TIMER was used to calculated MDSC fraction and correlation with LGALS8expression in the indicated types of tumors from the TCGA dataset. (D) Schematic procedure of phagocytosis assay. (E) Phagocytosis assay (FC) shows that Galectin-8 inhibits the ability of phagocytosis. F. Transcriptome analysis of CD14+ monocytes exposed with Galectin-8. (F) Heatmap of transcriptome sequencing data. Samples of 2 groups including control and gal-8 were analyzed. (G) Volcano plot of transcriptome sequencing data. Significantly regulated genes were labeled.$
Antibody Lgals8 Mammalian Hek293 Aaf19370 1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+lgals8/Human+Galectin-8+%2F+LGALS8+Protein/us10261091-664-125-130
Average 90 stars, based on 1 article reviews

antibody lgals8 mammalian hek293 aaf19370 1 - by Bioz Stars, 2026-09

90/100 stars

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human galectin-8 antibody (Bio-Techne corporation)

Bio-Techne corporation human galectin-8 antibody
$(A) Data analysis with TIDE shows that LGALS8 plays an important role in T cell dysfunction in tumor microenvironment. (B) Spearman correlations between <t>Galectin-8</t> expression and TILs. (C) TIMER was used to calculated MDSC fraction and correlation with LGALS8expression in the indicated types of tumors from the TCGA dataset. (D) Schematic procedure of phagocytosis assay. (E) Phagocytosis assay (FC) shows that Galectin-8 inhibits the ability of phagocytosis. F. Transcriptome analysis of CD14+ monocytes exposed with Galectin-8. (F) Heatmap of transcriptome sequencing data. Samples of 2 groups including control and gal-8 were analyzed. (G) Volcano plot of transcriptome sequencing data. Significantly regulated genes were labeled.$
Human Galectin 8 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+lgals8/Human+Galectin-8+Antibody/custom%40af1305%4029325545
Average 94 stars, based on 1 article reviews

human galectin-8 antibody - by Bioz Stars, 2026-09

94/100 stars

Buy from Supplier

human galectin-8 / lgals8 protein (Sino Biological)

Sino Biological human galectin-8 / lgals8 protein
$(A) Data analysis with TIDE shows that LGALS8 plays an important role in T cell dysfunction in tumor microenvironment. (B) Spearman correlations between <t>Galectin-8</t> expression and TILs. (C) TIMER was used to calculated MDSC fraction and correlation with LGALS8expression in the indicated types of tumors from the TCGA dataset. (D) Schematic procedure of phagocytosis assay. (E) Phagocytosis assay (FC) shows that Galectin-8 inhibits the ability of phagocytosis. F. Transcriptome analysis of CD14+ monocytes exposed with Galectin-8. (F) Heatmap of transcriptome sequencing data. Samples of 2 groups including control and gal-8 were analyzed. (G) Volcano plot of transcriptome sequencing data. Significantly regulated genes were labeled.$
Human Galectin 8 / Lgals8 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+lgals8/Human+Galectin-8+%2F+LGALS8+Protein/custom%4010301-hnae%4040707822
Average 92 stars, based on 1 article reviews

human galectin-8 / lgals8 protein - by Bioz Stars, 2026-09

92/100 stars

Buy from Supplier

galectin 8 lgals8 human shrna plasmid kit (OriGene)

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LGALS8 Human 4 unique 29mer shRNA constructs in lentiviral GFP vector

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recombinant human galectin 8 lgals8 protein (Elabscience)

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galectin 8 lgals8 nm 006499 human over expression lysate (OriGene)

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LGALS8 HEK293T cell transient overexpression lysate as WB positive control

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galectin 8 lgals8 nm 006499 human tagged orf clone (OriGene)

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LGALS8 Myc DDK tagged Human lectin galactoside binding soluble 8 LGALS8 transcript variant 1

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galectin 8 lgals8 nm 201543 human tagged orf clone (OriGene)

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LGALS8 Myc DDK tagged Human lectin galactoside binding soluble 8 LGALS8 transcript variant 2

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galectin 8 lgals8 nm 201544 human tagged orf clone (OriGene)

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Lenti ORF clone of Human lectin galactoside binding soluble 8 LGALS8 transcript variant 3 Myc DDK tagged

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Image Search Results

$(A) Data analysis with TIDE shows that LGALS8 plays an important role in T cell dysfunction in tumor microenvironment. (B) Spearman correlations between Galectin-8 expression and TILs. (C) TIMER was used to calculated MDSC fraction and correlation with LGALS8expression in the indicated types of tumors from the TCGA dataset. (D) Schematic procedure of phagocytosis assay. (E) Phagocytosis assay (FC) shows that Galectin-8 inhibits the ability of phagocytosis. F. Transcriptome analysis of CD14+ monocytes exposed with Galectin-8. (F) Heatmap of transcriptome sequencing data. Samples of 2 groups including control and gal-8 were analyzed. (G) Volcano plot of transcriptome sequencing data. Significantly regulated genes were labeled.$

Journal: bioRxiv

Article Title: Galectin-8 is a major ligand of LILRB4 prompting MDSC functions in the tumor microenvironment

doi: 10.1101/2022.07.27.501694

Figure Lengend Snippet: (A) Data analysis with TIDE shows that LGALS8 plays an important role in T cell dysfunction in tumor microenvironment. (B) Spearman correlations between Galectin-8 expression and TILs. (C) TIMER was used to calculated MDSC fraction and correlation with LGALS8expression in the indicated types of tumors from the TCGA dataset. (D) Schematic procedure of phagocytosis assay. (E) Phagocytosis assay (FC) shows that Galectin-8 inhibits the ability of phagocytosis. F. Transcriptome analysis of CD14+ monocytes exposed with Galectin-8. (F) Heatmap of transcriptome sequencing data. Samples of 2 groups including control and gal-8 were analyzed. (G) Volcano plot of transcriptome sequencing data. Significantly regulated genes were labeled.

Article Snippet: Reagents involving recombinant protein Galectin-8 (10301-HNAE; SinoBiological), APOE (APE-H5246; Acrobiosystems), CD3ε (10977-H02H; SinoBiological), CTLA-4 (CT4-H5255; Acrobiosystems), CD28 (CD8-H525a; Acrobiosystems), CD96 (TAE-H5252; Acrobiosystems), LAG-3 (LA3-H5255; Acrobiosystems), TIM-3 (TM3-H5258; Acrobiosystems), CD40 (CD0-5253; Acrobiosystems), ICOS (ICS-H5258; Acrobiosystems), OX40 (OX0-H5255; Acrobiosystems), TIGHT (TIT-H5254; Acrobiosystems), LY86 (10242-H02H; SinoBiological), LILRB4 (16742-H02H; SinoBiological), CD27 (CD7-H5254; Acrobiosystems), PD-1 (10377-H02H; SinoBiological), CD8b (11031-HCCH; SinoBiological) were also purchased from the indicated suppliers.

Techniques: Expressing, Phagocytosis Assay, Sequencing, Labeling

$(A) ELISA screening of potential Galectin-8 interactors revealed LILRB4. (B) 100X microscopic views of Immunofluorescence showing Galectin-8 binds to cell surface LILRB4. (C) CoIP of Galectin-8 and LILRB4. (D) Analysis of EC50 from ELISA test. Galectin-8 was coated by concentration scale, LILRB4-Fc was added to 1 μg/ml. (E) BLI assay showing the association-disassociation curve between Galectin-8 and LILRB4 with the kinetics constants. (F) Flow cytometry showing the fractions of MDSCs in PBMCs incubated with Gal-8, Gal-8 plus LILRB4 ectodomain, and APOE. (G) Flow cytometry showing the binding of Galectin8 to LILRB4 expressed on cell surface. (H) ELISA showing the binding between Galectin-8 and LILRB4 in presence of different concentrations of APOE.$

Journal: bioRxiv

Article Title: Galectin-8 is a major ligand of LILRB4 prompting MDSC functions in the tumor microenvironment

doi: 10.1101/2022.07.27.501694

Figure Lengend Snippet: (A) ELISA screening of potential Galectin-8 interactors revealed LILRB4. (B) 100X microscopic views of Immunofluorescence showing Galectin-8 binds to cell surface LILRB4. (C) CoIP of Galectin-8 and LILRB4. (D) Analysis of EC50 from ELISA test. Galectin-8 was coated by concentration scale, LILRB4-Fc was added to 1 μg/ml. (E) BLI assay showing the association-disassociation curve between Galectin-8 and LILRB4 with the kinetics constants. (F) Flow cytometry showing the fractions of MDSCs in PBMCs incubated with Gal-8, Gal-8 plus LILRB4 ectodomain, and APOE. (G) Flow cytometry showing the binding of Galectin8 to LILRB4 expressed on cell surface. (H) ELISA showing the binding between Galectin-8 and LILRB4 in presence of different concentrations of APOE.

Techniques: Enzyme-linked Immunosorbent Assay, Immunofluorescence, Concentration Assay, Flow Cytometry, Incubation, Binding Assay

(A) Phosphorylation of SHP1 was upregulated by the interaction of Galectin8 and LILRB4 in THP-1 cells. (B) THP-1 NFκB reporter cells cocultured with Galectin-8-overexpressed 293 cells rather than control 293 cells showed lower activation of NFκB signal. (C) Immune blotting demonstrates Gal-8 inhibited NFκB while activated STAT3 in THP-1 cells. (D) Nuclear protein extraction of NC/shLILRB4 THP-1 cells and immune blotting revealing effect of LILRB4 on NFκB and STAT3. (E) Immune blotting of human CD14+ cells. (F) Immune blotting of SHP-1 inhibitor, TPI, inhibited STAT3 phosphorylation in human CD14+ cells.

Journal: bioRxiv

Article Title: Galectin-8 is a major ligand of LILRB4 prompting MDSC functions in the tumor microenvironment

doi: 10.1101/2022.07.27.501694

Figure Lengend Snippet: (A) Phosphorylation of SHP1 was upregulated by the interaction of Galectin8 and LILRB4 in THP-1 cells. (B) THP-1 NFκB reporter cells cocultured with Galectin-8-overexpressed 293 cells rather than control 293 cells showed lower activation of NFκB signal. (C) Immune blotting demonstrates Gal-8 inhibited NFκB while activated STAT3 in THP-1 cells. (D) Nuclear protein extraction of NC/shLILRB4 THP-1 cells and immune blotting revealing effect of LILRB4 on NFκB and STAT3. (E) Immune blotting of human CD14+ cells. (F) Immune blotting of SHP-1 inhibitor, TPI, inhibited STAT3 phosphorylation in human CD14+ cells.

Techniques: Activation Assay, Protein Extraction

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